HER2 Monoclonal Antibody(11H9)

日期：2019-06-10 22:06　人气：
Catalog_no AM3045
Product_name HER2 Monoclonal Antibody(11H9)
Category 抗原抗体
Applications WB,IF,IHC-p
Reactivity Human,Mouse,Rat
Size 100μg/50μg
Price 2000.00/1100.00
Gene_name ERBB2
Protein_name Receptor tyrosine-protein kinase erbB-2
Human swiss prot no 2064
Human swiss prot no P04626
Mouse gene id 13866
Mouse swiss prot no P70424
Rat swiss prot no P06494
immunogen Synthetic Peptide of HER2
Specificity The antibody detects endogenous ErbB-2/HER-2 proteins.
Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source Mouse
Dilution WB: 1:2000-4000 IHC: 1:200 IF 1:200
Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability -20°C/1 year
Msds MSDS_Antibody.pdf
othername ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340
Molecular Weight 180KD
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    Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Mouse-spleen tissue. 1,HER2 Monoclonal Antibody(11H9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Rat-spleen tissue. 1,HER2 Monoclonal Antibody(11H9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) Hela, 2) Mouse Brain, diluted at 1:4000.
    IHC staining of human breast cancer tissue, diluted at 1:200.
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