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| Catalog_no | BD-PD400039 |
| Product_name | MERS-CoV Nucleoprotein / NP protein (His Tag) |
| Category | 冠状病毒产品 |
| Size | 100μg |
| Price | 8740.00 |
| Specificity | MERS-CoV |
| Source | Baculovirus-Insect Cells |
| Storage_stability | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| 稀释液/缓冲液 | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0.Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Please refer to the spe |
| 运输及保存条件 | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise |
| Observed_band | The recombinant Nucleoprotein MERS-CoV comprises 424 amino acids and has a predicted molecular mass of 46.51 kDa. |
| Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method |
| 生物活性 | Testing in progress |
| 预测N端 | A DNA sequence encoding the Nucleoprotein MERS-CoV (AFS88943.1) (Met1-Asp413) was fused with a polyhistidine tag at the C-terminus. |
| Purity | > 90 % as determined by SDS-PAGE |
| Instructions | 
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