Catalog_noA1029
Product_nameβ-actin Monoclonal Antibody(5B7)
Category抗原抗体
ApplicationsIF,WB,IHC-p
ReactivityHuman,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Insect
Size50μl/100μl
Price580.00/880.00
immunogenSynthetic Peptide of β-actin
SpecificityThe antibody detects endogenous β-actin protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionIF: 1:50-200 WB: 1:5000 IHC: 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration1 mg/ml
Observed_band43KD
Storage bufferPBS,pH 7.4,containing 0.02% sodium azide as Preservative and 50% Glycerol
Store at -20°C.Do not aliquot the antibody,For research use only
Backgroundβ-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of variousspecies and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin
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    Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.
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