Catalog_noA1032
Product_namePCNA Monoclonal Antibody(12D10)
Category抗原抗体
ApplicationsWB,IF,IHC-p
ReactivityHuman,Rat,Mouse
Size50μl/100μl
Price580.00/880.00
immunogenSynthetic Peptide of PCNA
SpecificityThe antibody detects endogenous PCNA protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:5000 IHC: 1:200 IF 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration1 mg/ml
Observed_band29KD
Storage bufferPBS,pH 7.4,containing 0.02% sodium azide as Preservative and 50% Glycerol
Store at -20°C.Do not aliquot the antibody,For research use only
BackgroundPCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is acofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesisduring DNA rep
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    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PCNA Monoclonal Antibody(12D10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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