Catalog_noBD-PD265968
Product_nameSARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD Antibody Titer Assay Kit
Size96T
CategoryELISA检测试剂盒
Price7500
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    Intended Use

    The kit applies for detecting the level of anti-SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD antibodies in serum.

    Principle of the assay

    The principle of the kit is indirect ELISA. SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombiant Protein has been pre-coated onto well plate strips. The samples or control antibody are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated Goat anti-Human IgG is added. Following a wash, the TMB substrate solution is loaded and color develops in proportion to the amount of antibodies. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm.

    Materials Provided

    Components 1 Kit (96 Tests)
    Microplate: Pre-coated with SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombiant Protein 1 Plate
    HRP-Goat anti-Human IgG 20 μL
    Anti-SARS-CoV-2 (2019-nCoV) Spike Antibody (Control) 20 μL
    20 × Dilution Buffer 5 mL
    20 × Wash Buffer 25 mL
    Color Reagent A 12.5 mL
    Color Reagent B 12.5 mL
    Stop Solution 8 mL
     

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