Catalog_noAC0067
Product_nameCleaved-Notch 1 (V1754) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IF,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameNOTCH1
Protein_nameNeurogenic locus notch homolog protein 1
Human swiss prot no4851
Human swiss prot noP46531
Mouse gene id18128
Mouse swiss prot noQ01705
Rat swiss prot noQ07008
immunogenThe antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784
SpecificityCleaved-Notch 1 (V1754) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWB 1:500-2000, IHC-p 1:50-300, IF 1:50-300
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameNOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1
Observed_band110KD
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    Immunofluorescence analysis of Human-lung-cancer tissue. 1,Cleaved-Notch 1 (V1754) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-lung-cancer tissue. 1,Cleaved-Notch 1 (V1754) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Human-lung-cancer tissue. 1,Cleaved-Notch 1 (V1754) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western Blot analysis of various cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1:500
    Western Blot analysis of KB cells using Cleaved-Notch 1 (V1754) Polyclonal Antibody diluted at 1:500
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