Catalog_noAM3055
Product_nameCK8 Monoclonal Antibody(8G8)
Category抗原抗体
ApplicationsIF,WB,IHC-p
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameKRT8
Protein_nameKeratin type II cytoskeletal 8
Human swiss prot no3856
Human swiss prot noP05787
Mouse gene id16691
Mouse swiss prot noP11679
Rat gene id25626
Rat swiss prot noQ10758
immunogenSynthetic Peptide of CK8
SpecificityThe antibody detects endogenous CK8 proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionIF: 1:50-200 WB: 1:2000-5000 IHC: 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameKRT8; CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Observed_band54KD
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    Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,CK8 Monoclonal Antibody(8G8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western blot analysis of 1) Hela, 2) Mouse Brain, diluted at 1:4000.
    IHC staining of human colon cancer tissue, diluted at 1:200.
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