Catalog_noAM3073
Product_nameFH Monoclonal Antibody(7F1)
Category抗原抗体
ApplicationsWB,IHC-p,IF
ReactivityHuman,Mouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameFH
Protein_nameFumarate hydratase, mitochondrial
Human swiss prot no2271
Human swiss prot noP07954
Mouse gene id14194
Mouse swiss prot noP97807
Rat swiss prot noP14408
immunogenSynthetic Peptide of FH
SpecificityThe antibody detects endogenous FH proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:3000 IF 1:200 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameFumarate hydratase, mitochondrial (Fumarase) (EC 4.2.1.2)
Observed_band50KD
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    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,FH Monoclonal Antibody(7F1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,FH Monoclonal Antibody(7F1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,FH Monoclonal Antibody(7F1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-liver-cancer tissue. 1,FH Monoclonal Antibody(7F1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-testis tissue. 1,FH Monoclonal Antibody(7F1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) 293T, 2) HepG2, 3) Hela, diluted at 1:3000.
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