Catalog_no | AM3074 |
Product_name | ABCB5 Monoclonal Antibody(11A2) |
Category | 抗原抗体 |
Applications | WB,IHC-p,IF, |
Reactivity | Human |
Size | 100μg/50μg |
Price | 2000.00/1100.00 |
Gene_name | ABCB5 |
Protein_name | ATP-binding cassette sub-family B member 5 |
Human swiss prot no | 340273 |
Human swiss prot no | Q2M3G0 |
immunogen | Synthetic Peptide of ABCB5 |
Specificity | The antibody detects endogenous ABCB5 proteins. |
Formulation | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
Source | Mouse |
Dilution | WB: 1:2000 IF 1:200 IHC 1:50-300 |
Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Storage_stability | -20°C/1 year |
Msds | MSDS_Antibody.pdf |
othername | ABCB5; ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5 |
Observed_band | 90KD |
Instructions | 查看与下载 |
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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,ABCB5 Monoclonal Antibody(11A2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | |
Immunofluorescence analysis of Human-liver-cancer tissue. 1,ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | |
Western blot analysis of 1) Hela, 2) 293T, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |