| Catalog_no | AM3081 |
| Product_name | CK17 Monoclonal Antibody(10A1) |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,IF,IP |
| Reactivity | Human |
| Size | 100μg/50μg |
| Price | 2000.00/1100.00 |
| Gene_name | KRT17 |
| Protein_name | Keratin type I cytoskeletal 17 |
| Human swiss prot no | 3872 |
| Human swiss prot no | Q04695 |
| Mouse gene id | 16667 |
| Mouse swiss prot no | Q9QWL7 |
| Rat gene id | 287702 |
| Rat swiss prot no | Q6IFU8 |
| immunogen | Synthetic Peptide of CK17 |
| Specificity | The antibody detects CK17 endogenous proteins. |
| Formulation | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Source | Mouse |
| Dilution | WB: 1:1000 IP:1:200 IF 1:200 IHC 1:50-300 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | KRT17; Keratin, type I cytoskeletal 17; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17 |
| Observed_band | 48KD |
| Instructions | 
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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,CK17 Monoclonal Antibody(10A1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunofluorescence analysis of Human-breast tissue. 1,CK17 Monoclonal Antibody(10A1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western blot analysis of 1) Hela, 2) MCF7, 3) 293T, diluted at 1:2000. |
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1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200 |
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