| Catalog_no | AM3083 |
| Product_name | IDE Monoclonal Antibody(3H4) |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,IF, |
| Reactivity | Human |
| Size | 100μg/50μg |
| Price | 2000.00/1100.00 |
| Gene_name | IDE |
| Protein_name | Insulin-degrading enzyme |
| Human swiss prot no | 3416 |
| Human swiss prot no | P14735 |
| Mouse gene id | |
| Mouse swiss prot no | Q9JHR7 |
| Rat gene id | 25700 |
| Rat swiss prot no | P35559 |
| immunogen | Synthetic Peptide of IDE |
| Specificity | The antibody detects endogenous IDE proteins. |
| Formulation | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Source | Mouse |
| Dilution | WB: 1:1000 IF 1:200 IHC 1:50-300 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | IDE; Insulin-degrading enzyme; Abeta-degrading protease; Insulin protease; Insulinase; Insulysin |
| Observed_band | 118KD |
| Instructions | 
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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,IDE Monoclonal Antibody(3H4) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunofluorescence analysis of Human-breast tissue. 1,IDE Monoclonal Antibody(3H4)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western blot analysis of 1) Hela, 2) HepG2, diluted at 1:2000 |
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