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Catalog_no	AM3085
Product_name	EFHD1 Monoclonal Antibody(3G2)
Category	抗原抗体
Applications	WB,IHC-p,IF
Reactivity	Human,Mouse,Rat
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	EFHD1
Protein_name	EF-hand domain-containing protein D1
Human swiss prot no	80303
Human swiss prot no	Q9BUP0
Mouse gene id	98363
Mouse swiss prot no	Q9D4J1
Rat swiss prot no
immunogen	Synthetic Peptide of EFHD1
Specificity	The antibody detects endogenous EFHD1 proteins.
Formulation	PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source	Mouse
Dilution	WB: 1:2000 IF: 1:100-200 IHC 1:50-300
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	EF-hand domain-containing protein D1 (EF-hand domain-containing protein 1) (Swiprosin-2)
Observed_band	27KD
Instructions	查看与下载
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	Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunofluorescence analysis of Mouse-lung tissue. 1,EFHD1 Monoclonal Antibody(3G2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western blot analysis of 1) Mouse spleen tissue, 2) Rat spleen tissue, diluted at 1:3000.
	IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.