Catalog_noAM3086
Product_nameXRCC4 Monoclonal Antibody(5C10)
Category抗原抗体
ApplicationsWB,IHC-p,IF,IP
ReactivityHuman
Size100μg/50μg
Price2000.00/1100.00
Gene_nameXRCC4
Protein_nameDNA repair protein XRCC4
Human swiss prot no7518
Human swiss prot noQ13426
Mouse gene id108138
Mouse swiss prot noQ924T3
Rat swiss prot no
immunogenSynthetic Peptide of XRCC4
SpecificityThe antibody detects endogenous XRCC4 proteins.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:2000 IP:1:200 IF 1:200 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameXRCC4; DNA repair protein XRCC4; X-ray repair cross-complementing protein 4
Observed_band38KD
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    Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,XRCC4 Monoclonal Antibody(5C10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-liver-cancer tissue. 1,XRCC4 Monoclonal Antibody(5C10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) Hela, 2) 293T, diluted at 1:3000.
    1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
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