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Catalog_no	AM3145
Product_name	Cleaved PARP Monoclonal Antibody(Mix)
Category	抗原抗体
Applications	IF,WB,IHC-p,
Reactivity	Human
Size	100μg/50μg
Price	2000.00/1100.00
Gene_name	PARP1
Protein_name	Poly [ADP-ribose] polymerase 1
Human swiss prot no	142
Human swiss prot no	P09874
Mouse gene id
Mouse swiss prot no	P11103
Rat gene id	25591
Rat swiss prot no	P27008
immunogen	Synthetic Peptide of Cleaved PARP
Specificity	The antibody detects endogenous pro and active PARP protein.
Formulation	PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source	Mouse
Dilution	IF: 1:50-200 WB: 1:2000-5000 IHC 1:50-300
Purification	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1
Observed_band	116KD,89KD
Instructions	查看与下载
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	Immunofluorescence analysis of human-liver-cancer tissue. 1,Cleaved PARP Monoclonal Antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Cleaved PARP Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Western blot analysis of Jurkat, diluted at 1:3000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).