| Catalog_no | AM3145 |
| Product_name | Cleaved PARP Monoclonal Antibody(Mix) |
| Category | 抗原抗体 |
| Applications | IF,WB,IHC-p, |
| Reactivity | Human |
| Size | 100μg/50μg |
| Price | 2000.00/1100.00 |
| Gene_name | PARP1 |
| Protein_name | Poly [ADP-ribose] polymerase 1 |
| Human swiss prot no | 142 |
| Human swiss prot no | P09874 |
| Mouse gene id | |
| Mouse swiss prot no | P11103 |
| Rat gene id | 25591 |
| Rat swiss prot no | P27008 |
| immunogen | Synthetic Peptide of Cleaved PARP |
| Specificity | The antibody detects endogenous pro and active PARP protein. |
| Formulation | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Source | Mouse |
| Dilution | IF: 1:50-200 WB: 1:2000-5000 IHC 1:50-300 |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1 |
| Observed_band | 116KD,89KD |
| Instructions | 
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Immunofluorescence analysis of human-liver-cancer tissue. 1,Cleaved PARP Monoclonal Antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Cleaved PARP Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western blot analysis of Jurkat, diluted at 1:3000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
