Catalog_noAM3378
Product_nameHAO1 Monoclonal Antibody(Mix)
Category抗原抗体
ApplicationsWB,IHC-p,IF,
ReactivityMouse,Rat
Size100μg/50μg
Price2000.00/1100.00
Gene_nameHAO1
Protein_nameHydroxyacid oxidase 1
Human swiss prot no54363
Human swiss prot noQ9UJM8
immunogenRecombinant Protein of HAO1
SpecificityThe antibody detects endogenous HAO1 protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:1000-2000 IF 1:200 IHC 1:50-300
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameHydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GOX
Observed_band41KD
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    Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,HAO1 Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunofluorescence analysis of Human-appendix tissue. 1,HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of Mouse-spleen tissue. 1,HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.
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