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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,Survivin Monoclonal Antibody(Mix) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western blot analysis of 1) Hela, 2) 293, 3) PC12 using Survivin Monoclonal Antibody. |
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Immunohistochemical analysis of paraffin-embedded human colon caricnoma using Survivin Monoclonal Antibody. |
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Western blot detection of Survivin in human breast cancer cell line MCF-7(A) and Cal51 (B) using Survivin mouse mAb (1:1000 diluted).Predicted band size: 16kDa.Observed band size:16kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Survivin Mouse mAb diluted at 1:200 (4°,overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital |
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Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |