Catalog_noAP0008
Product_nameAkt1 (phospho Thr450) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameAKT1
Protein_nameRAC-alpha serine/threonine-protein kinase
Human swiss prot no207
Human swiss prot noP31749
Mouse gene id11651
Mouse swiss prot noP31750
Rat gene id24185
Rat swiss prot noP47196
immunogenThe antiserum was produced against synthesized peptide derived from human Akt1 around the phosphorylation site of Thr450. AA range:416-465
SpecificityPhospho-Akt1 (T450) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at T450.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameAKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Observed_band55KD
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    Western Blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000
    Western Blot analysis of 453 cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000
    Western Blot analysis of HEPG2 KB A549 using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000
    Western blot analysis of various lysis using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
    The picture was kindly provided by our customer,antibody was diluted at 1:500
    Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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