Catalog_no | AP0093 |
Product_name | eIF2α (phospho Ser51) Polyclonal Antibody |
Category | 抗原抗体 |
Applications | IF,WB,IHC-p,ELISA |
Reactivity | Human,Mouse,Rat |
Size | 100μg/50μg/20μg |
Price | 2200.00/1200.00/560.00 |
Gene_name | EIF2S1 |
Protein_name | Eukaryotic translation initiation factor 2 subunit 1 |
Human swiss prot no | 1965 |
Human swiss prot no | P05198 |
Mouse gene id | 13665 |
Mouse swiss prot no | Q6ZWX6 |
Rat gene id | 54318 |
Rat swiss prot no | P68101 |
immunogen | The antiserum was produced against synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51. AA range:21-70 |
Specificity | Phospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51. |
Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Source | Rabbit |
Dilution | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Storage_stability | -20°C/1 year |
Msds | MSDS_Antibody.pdf |
othername | EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha |
Observed_band | 38KD |
Instructions | 查看与下载 |
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Immunofluorescence analysis of rat-heart tissue. 1,eIF2α (phospho Ser51) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | |
Immunofluorescence analysis of rat-heart tissue. 1,eIF2α (phospho Ser51) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | |
Western Blot analysis of various cells using Phospho-eIF2α (S51) Polyclonal Antibody diluted at 1:2000 | |
Western Blot analysis of 293 cells using Phospho-eIF2α (S51) Polyclonal Antibody diluted at 1:2000 |