Catalog_noAP0093
Product_nameeIF2α (phospho Ser51) Polyclonal Antibody
Category抗原抗体
ApplicationsIF,WB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameEIF2S1
Protein_nameEukaryotic translation initiation factor 2 subunit 1
Human swiss prot no1965
Human swiss prot noP05198
Mouse gene id13665
Mouse swiss prot noQ6ZWX6
Rat gene id54318
Rat swiss prot noP68101
immunogenThe antiserum was produced against synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51. AA range:21-70
SpecificityPhospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionIF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameEIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
Observed_band38KD
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    Immunofluorescence analysis of rat-heart tissue. 1,eIF2α (phospho Ser51) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of rat-heart tissue. 1,eIF2α (phospho Ser51) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western Blot analysis of various cells using Phospho-eIF2α (S51) Polyclonal Antibody diluted at 1:2000
    Western Blot analysis of 293 cells using Phospho-eIF2α (S51) Polyclonal Antibody diluted at 1:2000
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