Catalog_noAP0146
Product_nameIRS-1 (phospho Ser307) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat,Monkey
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameIRS1
Protein_nameInsulin receptor substrate 1
Human swiss prot no3667
Human swiss prot noP35568
Mouse gene id16367
Mouse swiss prot noP35569
Rat gene id25467
Rat swiss prot noP35570
immunogenSynthesized phospho-peptide around the phosphorylation site of human IRS-1 (phospho Ser307)
SpecificityPhospho-IRS-1 (S307) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S307.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameIRS1; Insulin receptor substrate 1; IRS-1
Observed_band180KD,130KD
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    Western Blot analysis of various cells using Phospho-IRS-1 (S307) Polyclonal Antibody diluted at 1:1000
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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