Catalog_noAP0166
Product_nameMEK-1 (phospho Thr292) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameMAP2K1
Protein_nameDual specificity mitogen-activated protein kinase kinase 1
Human swiss prot no5604
Human swiss prot noQ02750
Mouse gene id26395
Mouse swiss prot noP31938
Rat gene id170851
Rat swiss prot noQ01986
immunogenThe antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Thr291. AA range:261-310
SpecificityPhospho-MEK-1 (T292) Polyclonal Antibody detects endogenous levels of MEK-1 protein only when phosphorylated at T292.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameMAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1
Observed_band43KD
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    Western Blot analysis of 293 cells using Phospho-MEK-1 (T292) Polyclonal Antibody diluted at 1:1000
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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