| Catalog_no | AP0212 |
| Product_name | p53 (phospho Thr18) Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | IF,WB,IHC-p,ELISA |
| Reactivity | Human,Mouse,Rat |
| Size | 100μg/50μg/20μg |
| Price | 2200.00/1200.00/560.00 |
| Gene_name | TP53 |
| Protein_name | Cellular tumor antigen p53 |
| Human swiss prot no | 7157 |
| Human swiss prot no | P04637 |
| Mouse gene id | 22059 |
| Mouse swiss prot no | P02340 |
| Rat gene id | 24842 |
| Rat swiss prot no | P10361 |
| immunogen | The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Thr18. AA range:1-50 |
| Specificity | Phospho-p53 (T18) Polyclonal Antibody detects endogenous levels of p53 protein only when phosphorylated at T18. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | IF: 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53 |
| Observed_band | 46KD |
| Instructions | 
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-if-92.jpg) |
Immunofluorescence analysis of rat-lung tissue. 1,p53 (phospho Thr18) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
-if-93.jpg) |
Immunofluorescence analysis of rat-lung tissue. 1,p53 (phospho Thr18) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
poly-ihc-human-lung-cancer.jpg) |
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,p53 (phospho Thr18) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western Blot analysis of various cells using Phospho-p53 (T18) Polyclonal Antibody diluted at 1：2000 |
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Western Blot analysis of customer's using Phospho-p53 (T18) Polyclonal Antibody. Antibody was diluted at 1:2000 |
