Catalog_noAP0229
Product_namePKC ζ (phospho Thr410) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_namePRKCZ
Protein_nameProtein kinase C zeta type
Human swiss prot no5590
Human swiss prot noQ05513
Mouse gene id18762
Mouse swiss prot noQ02956
Rat gene id25522
Rat swiss prot noP09217
immunogenThe antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr410. AA range:376-425
SpecificityPhospho-PKC ζ (T410) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T410.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernamePRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta
Observed_band70KD
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    Western Blot analysis of various cells using Phospho-PKC ζ (T410) Polyclonal Antibody diluted at 1:1000
    Western Blot analysis of 823 cells using Phospho-PKC ζ (T410) Polyclonal Antibody diluted at 1:1000
    Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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