Catalog_noAP0239
Product_nameRaf-1 (phospho Ser259) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat,Monkey
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameRAF1
Protein_nameRAF proto-oncogene serine/threonine-protein kinase
Human swiss prot no5895
Human swiss prot noP04049
Mouse gene id110157
Mouse swiss prot noQ99N57
Rat gene id24703
Rat swiss prot noP11345
immunogenThe antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser259. AA range:225-274
SpecificityPhospho-Raf-1 (S259) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S259.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameRAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Observed_band73KD
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    Western Blot analysis of various cells using Phospho-Raf-1 (S259) Polyclonal Antibody diluted at 1:2000
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