Catalog_noAP0251
Product_nameStat3 (phospho Tyr705) Polyclonal Antibody
Category抗原抗体
ApplicationsIF,WB,IHC-p,IP,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameSTAT3
Protein_nameSignal transducer and activator of transcription 3
Human swiss prot no6774
Human swiss prot noP40763
Mouse gene id20848
Mouse swiss prot noP42227
Rat gene id25125
Rat swiss prot noP52631
immunogenThe antiserum was produced against synthesized peptide derived from human STAT3 around the phosphorylation site of Tyr705. AA range:672-721
SpecificityPhospho-Stat3 (Y705) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at Y705.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionIF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameSTAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Observed_band88KD
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    Immunofluorescence analysis of mouse-spleen tissue. 1,Stat3 (phospho Tyr705) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of mouse-spleen tissue. 1,Stat3 (phospho Tyr705) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Western Blot analysis of our customer's samples using primary antibody diluted at 1:500, The picture was kindly provided by our customer.. Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
    Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Stat3 (phospho Tyr705) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,Stat3 (phospho Tyr705) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Stat3 (phospho Tyr705) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western Blot analysis of various cells using Phospho-Stat3 (Y705) Polyclonal Antibody diluted at 1:2000
    Western Blot analysis of KB cells using Phospho-Stat3 (Y705) Polyclonal Antibody diluted at 1:2000
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