Catalog_noAP0372
Product_nameEg5 (phospho Thr926) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameKIF11
Protein_nameKinesin-like protein KIF11
Human swiss prot no3832
Human swiss prot noP52732
immunogenThe antiserum was produced against synthesized peptide derived from human KIF11/Eg5 around the phosphorylation site of Thr926. AA range:892-941
SpecificityPhospho-Eg5 (T926) Polyclonal Antibody detects endogenous levels of Eg5 protein only when phosphorylated at T926.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameKIF11; EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5
Observed_band119KD
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    Western Blot analysis of COLO205 cells using Phospho-Eg5 (T926) Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
    Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using Phospho-Eg5 (T926) antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
    Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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