Catalog_noAP0578
Product_nameShc (phospho Tyr349) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameSHC1
Protein_nameSHC-transforming protein 1
Human swiss prot no6464
Human swiss prot noP29353
Mouse gene id20416
Mouse swiss prot noP98083
Rat gene id85385
Rat swiss prot noQ5M824
immunogenThe antiserum was produced against synthesized peptide derived from human Shc around the phosphorylation site of Tyr349. AA range:315-364
SpecificityPhospho-Shc (Y349) Polyclonal Antibody detects endogenous levels of Shc protein only when phosphorylated at Y349.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameSHC1; SHC; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1
Observed_band66(p66 isoform), 52(p52 isoform), 46(p46 isoform)
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    Western Blot analysis of various cells using Phospho-Shc (Y349) Polyclonal Antibody diluted at 1:1000
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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