Catalog_noAP0597
Product_nameC/EBP α (phospho Ser21) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameCEBPA
Protein_nameCCAAT/enhancer-binding protein alpha
Human swiss prot no1050
Human swiss prot noP49715
Mouse gene id12606
Mouse swiss prot noP53566
Rat gene id24252
Rat swiss prot noP05554
immunogenThe antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21. AA range:6-55
SpecificityPhospho-C/EBP α (S21) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at S21.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameCEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha
Observed_band45KD
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    Western Blot analysis of various cells using Phospho-C/EBP α (S21) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
    Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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