Catalog_noAP0635
Product_nameGRF-1 (phospho Tyr1105) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameARHGAP35
Protein_nameRho GTPase-activating protein 35
Human swiss prot no2909
Human swiss prot noQ9NRY4
Mouse gene id232906
Mouse swiss prot noQ91YM2
Rat swiss prot noP81128
immunogenThe antiserum was produced against synthesized peptide derived from human GRF-1 around the phosphorylation site of Tyr1105. AA range:1071-1120
SpecificityPhospho-GRF-1 (Y1105) Polyclonal Antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Y1105.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameARHGAP35; GRF1; GRLF1; KIAA1722; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A
Observed_band160KD
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    Western Blot analysis of 3T3 cells using Phospho-GRF-1 (Y1105) Polyclonal Antibody
    Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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