Catalog_noAP0857
Product_nameHSP90β (phospho Ser254) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat,Monkey
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameHSP90AB1
Protein_nameHeat shock protein HSP 90-beta
Human swiss prot no3326
Human swiss prot noP08238
Mouse gene id15516
Mouse swiss prot noP11499
Rat gene id301252
Rat swiss prot noP34058
immunogenThe antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. AA range:226-275
SpecificityPhospho-HSP90β (S254) Polyclonal Antibody detects endogenous levels of HSP90β protein only when phosphorylated at S254.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameHSP90AB1; HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84
Observed_band90KD
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    Western Blot analysis of various cells using Phospho-HSP90β (S254) Polyclonal Antibody
    Western Blot analysis of HepG2 cells using Phospho-HSP90β (S254) Polyclonal Antibody
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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