Catalog_noAP0859
Product_nameCytokeratin 18 (phospho Ser33) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameKRT18
Protein_nameKeratin type I cytoskeletal 18
Human swiss prot no3875
Human swiss prot noP05783
Mouse gene id16668
Mouse swiss prot noP05784
Rat gene id294853
Rat swiss prot noQ5BJY9
immunogenThe antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser33. AA range:1-50
SpecificityPhospho-Cytokeratin 18 (S33) Polyclonal Antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at S33.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameKRT18; CYK18; PIG46; Keratin; type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Observed_band47KD
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    Western Blot analysis of various cells using Phospho-Cytokeratin 18 (S33) Polyclonal Antibody diluted at 1:2000
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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