Catalog_noAP0867
Product_nameCot (phospho Thr290) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameMAP3K8
Protein_nameMitogen-activated protein kinase kinase kinase 8
Human swiss prot no1326
Human swiss prot noP41279
Mouse gene id26410
Mouse swiss prot noQ07174
Rat gene id116596
Rat swiss prot noQ63562
immunogenThe antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290. AA range:256-305
SpecificityPhospho-Cot (T290) Polyclonal Antibody detects endogenous levels of Cot protein only when phosphorylated at T290.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameMAP3K8; COT; ESTF; Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; TPL-2
Observed_band60KD
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    Western Blot analysis of various cells using Phospho-Cot (T290) Polyclonal Antibody
    Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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