Catalog_noAP1004
Product_nameEmt (phospho Tyr512) Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,ELISA
ReactivityHuman,Mouse
Size100μg/50μg/20μg
Price2200.00/1200.00/560.00
Gene_nameITK
Protein_nameTyrosine-protein kinase ITK/TSK
Human swiss prot no3702
Human swiss prot noQ08881
Mouse gene id16428
Mouse swiss prot noQ03526
immunogenThe antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512. AA range:478-527
SpecificityPhospho-Emt (Y512) Polyclonal Antibody detects endogenous levels of Emt protein only when phosphorylated at Y512.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionImmunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameITK; EMT; LYK; Tyrosine-protein kinase ITK/TSK; Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk
Observed_band72KD
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    Western blot analysis of 293 using p-Emt (Y512) antibody.
    Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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