| Catalog_no | AT2163 |
| Product_name | Histone H3 Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,IF,ELISA |
| Reactivity | Human,Mouse,Rat |
| Size | 100μg/50μg/20μg |
| Price | 2000.00/1100.00/500.00 |
| Gene_name | HIST1H3A |
| Protein_name | Histone H3.1 |
| Human swiss prot no | 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968 |
| Human swiss prot no | P68431/Q71DI3/P84243 |
| Rat gene id | 291159/100361558 |
| Rat swiss prot no | Q6LED0/P84245 |
| immunogen | The antiserum was produced against synthesized peptide derived from human Histone H3.1. AA range:1-50 |
| Specificity | Histone H3 Polyclonal Antibody detects endogenous levels of Histone H3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3 |
| Observed_band | 20KD |
| Instructions | 
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Western Blot analysis of various cells using Histone H3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
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Western Blot analysis of MCF-7 cells using Histone H3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
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Western Blot analysis of customer's (cat sample) using Histone H3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). |
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Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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