Catalog_noAT2973
Product_nameNa+/K+-ATPase α1 Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameATP1A1
Protein_nameSodium/potassium-transporting ATPase subunit alpha-1
Human swiss prot no476
Human swiss prot noP05023
Mouse gene id11928
Mouse swiss prot noQ8VDN2
Rat gene id24211
Rat swiss prot noP06685
immunogenThe antiserum was produced against synthesized peptide derived from human ATPase. AA range:5-54
SpecificityNa+/K+-ATPase α1 Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1
Observed_band112KD
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    Western Blot analysis of various cells using Na+/K+-ATPase α1 Polyclonal Antibody diluted at 1:1000
    Western Blot analysis of 293 cells using Na+/K+-ATPase α1 Polyclonal Antibody diluted at 1:1000
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
    Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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