Catalog_noAT3169
Product_nameNOS2 Polyclonal Antibody
Category抗原抗体
ApplicationsIF,WB,IHC-p,ELISA
ReactivityHuman,Mouse,Rat
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameNOS2
Protein_nameNitric oxide synthase inducible
Human swiss prot no4843
Human swiss prot noP35228
Mouse gene id18126
Mouse swiss prot noP29477
Rat gene id24599
Rat swiss prot noQ06518
immunogenThe antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166
SpecificityNOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionIF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameNOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Observed_band131KD
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    Immunofluorescence analysis of rat-kidney tissue. 1,NOS2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of rat-kidney tissue. 1,NOS2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of mouse-lung tissue. 1,NOS2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of mouse-lung tissue. 1,NOS2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of mouse-kidney tissue. 1,NOS2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of mouse-kidney tissue. 1,NOS2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,NOS2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western Blot analysis of various cells using NOS2 Polyclonal Antibody diluted at 1:500
    Western Blot analysis of SH-SY5Y cells using NOS2 Polyclonal Antibody diluted at 1:500
    Western blot analysis of 3T3 lysis using NOS2 antibody. Antibody was diluted at 1:500
    Western blot analysis of 3T3 lysis using NOS2 antibody. Antibody was diluted at 1:500
    Western blot analysis of 3T3 lysis using NOS2 antibody. Antibody was diluted at 1:500
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