| Catalog_no | AT3501 |
| Product_name | p27 Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | WB,IHC-p,ELISA |
| Reactivity | Human |
| Size | 100μg/50μg/20μg |
| Price | 2000.00/1100.00/500.00 |
| Gene_name | CDKN1B |
| Protein_name | Cyclin-dependent kinase inhibitor 1B |
| Human swiss prot no | 1027 |
| Human swiss prot no | P46527 |
| immunogen | The antiserum was produced against synthesized peptide derived from human p27 Kip1. AA range:149-198 |
| Specificity | p27 Polyclonal Antibody detects endogenous levels of p27 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1 |
| Observed_band | 27KD |
| Instructions | 
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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,p27 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1,p27 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Western Blot analysis of various cells using p27 Polyclonal Antibody diluted at 1：500 |
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Western Blot analysis of COLO205 cells using p27 Polyclonal Antibody diluted at 1：500 |
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