Catalog_noAT3817
Product_namePOLR3E Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman,Mouse
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_namePOLR3E
Protein_nameDNA-directed RNA polymerase III subunit RPC5
Human swiss prot no55718
Human swiss prot noQ9NVU0
Mouse gene id26939
Mouse swiss prot noQ9CZT4
immunogenThe antiserum was produced against synthesized peptide derived from human RPC5. AA range:191-240
SpecificityPOLR3E Polyclonal Antibody detects endogenous levels of POLR3E protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernamePOLR3E; KIAA1452; DNA-directed RNA polymerase III subunit RPC5; RNA polymerase III subunit C5; DNA-directed RNA polymerase III 80 kDa polypeptide
Observed_band80KD
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    Western Blot analysis of various cells using POLR3E Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
    Western Blot analysis of JK cells using POLR3E Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
    Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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