Catalog_noAT4915
Product_nameXPG Polyclonal Antibody
Category抗原抗体
ApplicationsWB,IHC-p,IF,ELISA
ReactivityHuman
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameERCC5
Protein_nameDNA repair protein complementing XP-G cells
Human swiss prot no2073
Human swiss prot noP28715
immunogenThe antiserum was produced against synthesized peptide derived from human ERCC5. AA range:131-180
SpecificityXPG Polyclonal Antibody detects endogenous levels of XPG protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernameERCC5; ERCM2; XPG; XPGC; DNA repair protein complementing XP-G cells; DNA excision repair protein ERCC-5; Xeroderma pigmentosum group G-complementing protein
Observed_band130KD
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    Western Blot analysis of various cells using XPG Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
    Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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