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常规抗体

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Catalog_no	AT5138
Product_name	IL-10 Polyclonal Antibody
Category	抗原抗体
Applications	WB,IHC-p,ELISA
Reactivity	Human
Size	100μg/50μg/20μg
Price	2000.00/1100.00/500.00
Gene_name	IL10
Protein_name	Interleukin-10
Human swiss prot no	3586
Human swiss prot no	P22301
immunogen	The antiserum was produced against synthesized peptide derived from the Internal region of human IL10. AA range:71-120
Specificity	IL-10 Polyclonal Antibody detects endogenous levels of IL-10 protein.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source	Rabbit
Dilution	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration	1 mg/ml
Storage_stability	-20°C/1 year
Msds	MSDS_Antibody.pdf
othername	IL10; Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF
Observed_band	20KD
Instructions	查看与下载
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	Western Blot analysis of THP-1, Romas, HepG2, SW480, SHSY5Y, Mouse liver cells using IL-10 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
	Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:100
	Immunohistochemical analysis of paraffin-embedded Human Cerebral cortex. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
	Immunohistochemical analysis of paraffin-embedded Human Cerebral cortex. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
	Immunohistochemical analysis of paraffin-embedded Human Cerebral cortex. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
	Immunohistochemical analysis of paraffin-embedded Human lymphoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
	Immunohistochemical analysis of paraffin-embedded Human lymphoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
	Immunohistochemical analysis of paraffin-embedded Human lymphoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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