| Catalog_no | AT5198 |
| Product_name | Cdk4 Polyclonal Antibody |
| Category | 抗原抗体 |
| Applications | IF,WB,IHC-p,ELISA |
| Reactivity | Human,Mouse,Rat |
| Size | 100μg/50μg/20μg |
| Price | 2000.00/1100.00/500.00 |
| Gene_name | CDK4 |
| Protein_name | Cyclin-dependent kinase 4 |
| Human swiss prot no | 1019 |
| Human swiss prot no | P11802 |
| Mouse gene id | 12567 |
| Mouse swiss prot no | P30285 |
| Rat gene id | 94201 |
| Rat swiss prot no | P35426 |
| immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human CDK4. AA range:1-50 |
| Specificity | Cdk4 Polyclonal Antibody detects endogenous levels of Cdk4 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | Rabbit |
| Dilution | IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage_stability | -20°C/1 year |
| Msds | MSDS_Antibody.pdf |
| othername | CDK4; Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3 |
| Observed_band | 35KD |
| Instructions | 
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Immunofluorescence analysis of human-stomach tissue. 1,Cdk4 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Immunofluorescence analysis of rat-lung tissue. 1,Cdk4 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Western Blot analysis of MCF7, K562 cells using Cdk4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 |
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100 |
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100 |
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100 |
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Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100 |
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Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100 |
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