Catalog_noAT5743
Product_nameCaspase-1 Polyclonal Antibody
Category抗原抗体
ApplicationsIF,WB,IHC-p,ELISA
ReactivityHuman,Rat
Size100μg/50μg/20μg
Price2000.00/1100.00/500.00
Gene_nameCASP1 IL1BC IL1BCE
Protein_nameCaspase-1
Human swiss prot no834
Human swiss prot noP29466
immunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human CASP1. AA range:350-400
SpecificityCaspase-1 Polyclonal Antibody detects endogenous levels of Caspase-1
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SourceRabbit
DilutionIF: 1:50-200 WB 1:500-2000, IHC 1:50-300, ELISA 1:10000-20000
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration1 mg/ml
Storage_stability-20°C/1 year
MsdsMSDS_Antibody.pdf
othernamecaspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Observed_band35KD
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    Immunofluorescence analysis of rat-lung tissue. 1,Caspase-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunofluorescence analysis of rat-lung tissue. 1,Caspase-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Caspase-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Caspase-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Caspase-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Caspase-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    Western Blot analysis of 293T Hela MCF-7 Hela-UV MCF-7-UV KB-UV cells using Caspase-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
    Immunohistochemical analysis of paraffin-embedded Human-skin, antibody was diluted at 1:100
    Immunohistochemical analysis of paraffin-embedded Human-skin, antibody was diluted at 1:100
    Immunohistochemical analysis of paraffin-embedded Human-lung, antibody was diluted at 1:100
    Immunohistochemical analysis of paraffin-embedded Human-lung, antibody was diluted at 1:100
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