Catalog_noA1031
Product_nameβ-Tubulin Monoclonal Antibody(5G3)
Category抗原抗体
ApplicationsWB,IF,IHC-p
ReactivityHuman,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,sheep,Insect,Yeast
Size50μl/100μl
Price580.00/880.00
immunogenSynthetic Peptide of β-Tubulin
SpecificityThe antibody detects endogenous β-tubulin protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:5000 IHC: 1:200 IF 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration1 mg/ml
Observed_band55KD
Storage bufferPBS,pH 7.4,containing 0.02% sodium azide as Preservative and 50% Glycerol
Store at -20°C.Do not aliquot the antibody,For research use only
BackgroundMicrotubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. Theyconsist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are usef
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    Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,β-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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