Catalog_noA1030
Product_nameGAPDH Monoclonal Antibody(2B8)
Category抗原抗体
ApplicationsWB,IF,IHC-p
ReactivityHuman,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Pig,sheep,Insect,Yeast
Size50μl/100μl
Price580.00/880.00
immunogenSynthetic Peptide of GAPDH
SpecificityThe antibody detects endogenous GAPDH protein.
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
SourceMouse
DilutionWB: 1:5000 IHC: 1:200 IF 1:200
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration1 mg/ml
Observed_band37KD
Storage bufferPBS,pH 7.4,containing 0.02% sodium azide as Preservative and 50% Glycerol
Store at -20°C.Do not aliquot the antibody,For research use only
BackgroundGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved inglycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful asloading controls
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    Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.
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