ELISA试剂盒

Intended Use

The kit applies for detecting the level of anti-SARS-CoV-2 (2019-nCoV) Spike RBD antibodies in mouse serum.

Principle of the assay

The principle of the kit is indirect ELISA. SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombiant Protein has been pre-coated onto well plate strips. The samples or control antibody are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated Rabbit anti-Mouse IgG is added, producing an complex "antigen-antibody-labeled antibody". Following a wash, the TMB substrate solution is loaded and color develops in proportion to the amount of antibodies. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm.

Materials Provided